You might do a lot of usability testing on your web site but you still might lose up to 10% of your audience because of some simple mistakes with colors. Specifically, your web site may be designed in a way that doesn't work well for people with colorblindness. Generally the fix is quite simple: be sure to provide excellent contrast between your various web page elements.
Follansbee, Todd. WebWord (2001). Design>Web Design>Accessibility>Color